Bioethanol production from grass pea and wild oat hydrolysates using S. cerevisiae ETP53, K. marxianus ETP87, and P. fermentans ETP22

Abstract Conversion of lignocellulosic biomass into bioethanol is essential to reduce dependency on fossil fuels. After the grass pea and wild oat straws were characterized, they hydrolyzed by live fungi (in situ), crude fungal enzymes, chemical methods (sodium hydroxide sulphuric acids at 0.5, 1, 2, 3%). The used for in situ degradation enzymes Aspergillus niger JMC22344, Trichoderma reesei JMC22, Pleurotus ostreatus M2191, sajor-caju M2145. Furfural content hydrolysates was analyzed GC–MS. potential activated charcoal, overliming, sequential charcoal-overliming furfural acid evaluated. fermented S. cerevisiae ETP53, K. marxianus ETP87, P. fermentans ETP22. Sulphuric common (8.91 g/L) yielded higher sugars than NaOH (3.88 treated (8.14 superior terms released (4.21 g/L). M2191 liberated highest sugar (18.63 extracts T. JMC22676, A. ETP22344 23–77% reduced charcoal-overliming. Yeasts produced optimal ethanol from JMC22344. Generally, titer directly proportional amount released.